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MRPrimerW3
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Search Primer Pairs
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List of species to filter out:
Select search option:
Gene Symbol
Gene ID
NCBI Accession Number
Designing primers amplifying all given sequences
Search for:
Advanced Settings
Single filtering
Primer melting temperatures (T
m
)
Min
Max
GC content (%)
Min
Max
Primer length (bp)
Min
Max
Self-complementarity
Max
Self-complementarity at 3' end
Max
Contiguous repeats (bp)
Max
End Stability (kcal/mol)
Max
Hairpin
Max
Pair filtering
Product size (bp)
Min
Max
Length difference between the two primers
Max
Melting temperature difference between the two primers
Max
Pair-complementarity
Max
3' end pair-complementarity
Max
Submit
Example 1 (Gene search)
Example 2 (batch search)
Example 3 (multi-target search)
Example 4 (species-specific search)