Search Primer Pairs

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List of species to filter out:



Advanced Settings 

Single filtering

Primer melting temperatures (Tm)
 
GC content (%)
 
Primer length (bp)
 
Self-complementarity
Self-complementarity at 3' end
Contiguous repeats (bp)
End Stability (kcal/mol)
Hairpin

Pair filtering

Product size (bp)
 
Length difference between the two primers
Melting temperature difference between the two primers
Pair-complementarity
3' end pair-complementarity